The World of SmArt PCR Reagents

Recombinant Taq DNA Polymerase is expressed in E.coli. Taq DNA Polymerase is most frequently used enzyme in PCR diagnostics. Requires a Hot Start modification to get excellent results. The best choice who appreciates the budget.

Features:

- human DNA-free

- extremely low content of DNA E.coli

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

M-MLV is a genetically modified Reverse Transcriptase from Moloney Murine Leukemia Virus. It is different from wild-type M-MuLV in structure, catalytic features and temperature optimum of activity. The enzyme possesses RNA- and DNA-dependent polymerase activity but lacks RNase H activity. Temperature optimum for M-MLV activity is 52°C. The enzyme remains active at temperatures up to 60 °C. M-MLV is able to synthesize first strand cDNA up to 10 kb and incorporate modified bases.

Features:

- optimal activity at 52°С

- thermostability till 60 °C

- RNAse H-free

- human DNA-free

- extremely low content of E.coli DNA

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

dNTPs is an integral component of any PCR. Obtained by biotechnological synthesis, don’t include inhibitors of PCR. All natural dNTPs are available (dATP, dGTP, dTTP, dCTP) and dUTP.

Features:

- PCR grade

- biotechnological synthesis

- availability dUTP

- human DNA-free

- extremely low content of E.coli DNA

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

Recombinant Taq DNA Polymerase with a Hot Start modification for excellent results. It available in two Hot Start modifications: with a thermolabile neutralizing antibody that blocks the polymerase activity prior to 39 °С or 56 °С  and with chemicals that required polymerase activation at 95 ^oC.  Hot Start technology improves yields by minimizing or eliminating primer dimer formation and non-specific amplification.

Features:

- activation of your choice: 39/56/95 ^oС

- human DNA-free

- extremely low content of DNA E.coli

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

WarmMMLV is modified inactivated M-MLV with increased processivity, activity, and resistance to inhibitors, lack of RNase H. WarmMMLV is activated at 40 ^oС that improves sensitivity of RT-PCR by minimizing or eliminating primer dimer formation and non-specific amplification.

Features:

- activation at 40 ^oС

- optimal activity at 52°С

- thermostability till 60 °C

- RNAse H-

- human DNA-free

- extremely low content of E.coli DNA

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

Recombinant Uracil-DNA Glycosylase (UDG) is expressed in E.coli. UDG is most frequently used enzyme in PCR anti-contamination system. UDG degrades amplicone containing deoxyuridine and increases the specificity of your PCR.

Features:

- human DNA-free

- extremely low content of DNA E.coli

- any concentration and packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

Ready to use PCR mixes include all needed components: Hot Start Taq DNA polymerase, Reaction Buffer, Mg²⁺, dNTPs, PCR enhancers and additives, storage stabilizers, anticontamination system, and fluorescent dyes. Sensitivity is 5 copies/reaction.

Features:

- activation of your choice: 39/56/95 ^oС

- inclusion of an anti-contamination system on your choice (UDG/dUTP)

- inclusion of dyes on your choice ROX and/or Eva Green

- adaptation of PCR mix under your task: TaqMan, SYBR Green, routine, long, direct, HF, etc.

- concentration of your choice: 2х, 5х, 10х.

- human DNA-free

- extremely low content of E.coli DNA

- any packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

 

Ready to use RT-PCR mixes for using in One Step RT-PCR include all needed components: Hot Start Taq DNA polymerase, WarmMMLV Reverse Transcriptase, Reaction Buffer, Mg²⁺, dNTPs, PCR enhancers and additives, storage stabilizers, anticontamination system, fluorescent dyes. Sensitivity is 5 copies/reaction.

Features:

- activation at 40 ^oС

- inclusion of an anti-contamination system on your choice (UDG/dUTP)

- inclusion of dyes on your choice ROX and/or Eva Green

- adaptation of PCR mix under your task: TaqMan, SYBR Green, routine, long, direct, HF, etc.

- human DNA-free

- extremely low content of E.coli DNA

- any packing convenient for you

- ambient temperature transportation

- shelf life up to 24 months

Recombinant Proteinase K is identical to the native protease originally isolated from the Tritirachium album. It belongs to family of serine proteases with broad substrate specificity. Proteinase K is active in a wide range of temperatures and buffers (optimal activity between 20 and 60°C and a pH between 7.5 and 12.0) even in the presence of EDTA and useful for the extraction of nucleic acids from various biological samples.

  Features:

- human DNA-free

- ambient temperature transportation

- shelf life up to 24 months